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A. Toluidine blue staining of ITS-induced ATDC5 cells at different time points (0, 7, 14 and 21 days). B. Immunocytochemistry staining <t>(COL2A1</t> and MMP-13) of ITS-induced ATDC5 cells on Days 0 and 1 (n = 5). C. Effects of SGD on chondrocyte-like cell proliferation (n = 6). D. Immunocytochemistry staining (COL2A1 and MMP-13) of IL-1β-induced chondrocyte-like cells after SGD-treated serum intervention: (a) blank group, (b) model group, and (c) experimental group (n = 5). Data are expressed as the mean±SD, the blank group vs. model or 14 day group, # p < 0.05, ## p < 0.01, ### p < 0.001, vs. the SGD, diacerein, experimental group * p < 0.05, ** p < 0.01, *** p < 0.001. (one-way ANOVA were used).
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A. Toluidine blue staining of ITS-induced ATDC5 cells at different time points (0, 7, 14 and 21 days). B. Immunocytochemistry staining <t>(COL2A1</t> and MMP-13) of ITS-induced ATDC5 cells on Days 0 and 1 (n = 5). C. Effects of SGD on chondrocyte-like cell proliferation (n = 6). D. Immunocytochemistry staining (COL2A1 and MMP-13) of IL-1β-induced chondrocyte-like cells after SGD-treated serum intervention: (a) blank group, (b) model group, and (c) experimental group (n = 5). Data are expressed as the mean±SD, the blank group vs. model or 14 day group, # p < 0.05, ## p < 0.01, ### p < 0.001, vs. the SGD, diacerein, experimental group * p < 0.05, ** p < 0.01, *** p < 0.001. (one-way ANOVA were used).
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A. Toluidine blue staining of ITS-induced ATDC5 cells at different time points (0, 7, 14 and 21 days). B. Immunocytochemistry staining (COL2A1 and MMP-13) of ITS-induced ATDC5 cells on Days 0 and 1 (n = 5). C. Effects of SGD on chondrocyte-like cell proliferation (n = 6). D. Immunocytochemistry staining (COL2A1 and MMP-13) of IL-1β-induced chondrocyte-like cells after SGD-treated serum intervention: (a) blank group, (b) model group, and (c) experimental group (n = 5). Data are expressed as the mean±SD, the blank group vs. model or 14 day group, # p < 0.05, ## p < 0.01, ### p < 0.001, vs. the SGD, diacerein, experimental group * p < 0.05, ** p < 0.01, *** p < 0.001. (one-way ANOVA were used).

Journal: PLOS ONE

Article Title: Identification of the key role of IL-17RB in the treatment of osteoarthritis with Shaoyao Gancao decoction: Verification based on RNA-seq and bioinformatics analysis

doi: 10.1371/journal.pone.0315913

Figure Lengend Snippet: A. Toluidine blue staining of ITS-induced ATDC5 cells at different time points (0, 7, 14 and 21 days). B. Immunocytochemistry staining (COL2A1 and MMP-13) of ITS-induced ATDC5 cells on Days 0 and 1 (n = 5). C. Effects of SGD on chondrocyte-like cell proliferation (n = 6). D. Immunocytochemistry staining (COL2A1 and MMP-13) of IL-1β-induced chondrocyte-like cells after SGD-treated serum intervention: (a) blank group, (b) model group, and (c) experimental group (n = 5). Data are expressed as the mean±SD, the blank group vs. model or 14 day group, # p < 0.05, ## p < 0.01, ### p < 0.001, vs. the SGD, diacerein, experimental group * p < 0.05, ** p < 0.01, *** p < 0.001. (one-way ANOVA were used).

Article Snippet: The sections were blocked with 5% goat serum (Zsbio, PRC) and then incubated with anti-rabbit COL2A1 and MMP-13 primary antibodies (Proteintech, USA) overnight at 4°C after antigen retrieval.

Techniques: Staining, Immunocytochemistry

A. Immunohistochemistry staining (COL2A1) of the tibial plateau of rats (n = 3). B. Immunohistochemistry staining (MMP-13) of the tibial plateau of rats (n = 3). C. OD value of immunohistochemistry staining (COL2A1 and MMP-13). Data are expressed as the mean±SD vs. the model group, #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the SGD or diacerein group * p < 0.05, ** p < 0.01, *** p < 0.001. (one-way ANOVA were used).

Journal: PLOS ONE

Article Title: Identification of the key role of IL-17RB in the treatment of osteoarthritis with Shaoyao Gancao decoction: Verification based on RNA-seq and bioinformatics analysis

doi: 10.1371/journal.pone.0315913

Figure Lengend Snippet: A. Immunohistochemistry staining (COL2A1) of the tibial plateau of rats (n = 3). B. Immunohistochemistry staining (MMP-13) of the tibial plateau of rats (n = 3). C. OD value of immunohistochemistry staining (COL2A1 and MMP-13). Data are expressed as the mean±SD vs. the model group, #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the SGD or diacerein group * p < 0.05, ** p < 0.01, *** p < 0.001. (one-way ANOVA were used).

Article Snippet: The sections were blocked with 5% goat serum (Zsbio, PRC) and then incubated with anti-rabbit COL2A1 and MMP-13 primary antibodies (Proteintech, USA) overnight at 4°C after antigen retrieval.

Techniques: Immunohistochemistry, Staining